Juventus missed out on the chance to wrap up the Serie A title as they fell to a surprise defeat at struggling Udinese.
Juve, who have been Italian champions for exactly 3,000 days, need to win one of their final three games to win their ninth title in a row.
They led through Matthijs de Ligt’s powerful drive.
But Ilija Nestorovski headed in an equaliser before Seko Fofana’s brilliant winner in injury time.
As Maurizio Sarri’s side pushed for a winner, Fofana ran from the halfway line, beat Alex Sandro for pace before cutting inside De Ligt and slotting the ball under Wojciech Szczesny.
Juventus only need three points to seal an unprecedented ninth consecutive Serie A title – but they are out of form.
They have only won one of their past five Serie A games, but the three teams below them – Atalanta, Inter Milan and Lazio – have only won five games between them in a combined 15 matches.
There are doubts about the future of manager Maurizio Sarri, who is hoping to win the first league title of his career.
Juve looked laboured at Udinese – who are not yet safe from relegation – with most of their 21 shots either speculative or from long range.
They play Sampdoria on Sunday (20:45 BST) – although could be champions before then if their rivals lose their weekend matches.
Match ends, Udinese 2, Juventus 1.
Second Half ends, Udinese 2, Juventus 1.
Paulo Dybala (Juventus) wins a free kick in the defensive half.
Foul by Rodrigo de Paul (Udinese).
Corner, Juventus. Conceded by Samir.
Attempt blocked. Douglas Costa (Juventus) left footed shot from the right side of the box is blocked.
Substitution, Udinese. Sebastien De Maio replaces Rodrigo Becão because of an injury.
Goal! Udinese 2, Juventus 1. Seko Fofana (Udinese) left footed shot from the centre of the box to the bottom left corner. Assisted by Rodrigo Becão with a headed pass following a fast break.
Matthijs de Ligt (Juventus) wins a free kick in the defensive half.
Foul by Ken Sema (Udinese).
Corner, Udinese. Conceded by Adrien Rabiot.
Foul by Blaise Matuidi (Juventus).
Rodrigo Becão (Udinese) wins a free kick in the defensive half.
Blaise Matuidi (Juventus) wins a free kick in the attacking half.
Foul by Stefano Okaka (Udinese).
Attempt missed. Paulo Dybala (Juventus) header from the centre of the box misses to the left. Assisted by Juan Cuadrado with a cross.
Attempt missed. Rodrigo de Paul (Udinese) right footed shot from outside the box is high and wide to the right. Assisted by Seko Fofana.
Attempt blocked. Seko Fofana (Udinese) left footed shot from outside the box is blocked. Assisted by Ilija Nestorovski.
Attempt missed. Stefano Okaka (Udinese) header from the centre of the box is too high. Assisted by Rodrigo de Paul with a cross following a set piece situation.
Juan Cuadrado (Juventus) is shown the yellow card for a bad foul.
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